Objective and Conclusion In Lab 3B, we performed paper chromatography tests with green, a primary colour, and an unknown colour. In this lab, we performed Rf value calculations. Through this process we learned that many components correspond to a certain Rf value. This value is the quotient of the distance from the starting line to the sample spot, and from the starting line to the solvent front, being the dividend and divisor, respectively.
Results From Actual Experiment
On our chromatography paper testing green food colouring, it split into two colours: yellow and blue. This confirms that green is a mixture of the components, yellow and blue. In art, this is well known: that the secondary colour "green" is a combination of the primary colours "yellow" and "blue". Below is a video of a very similar experiment.
Paper Chromatography in the Real World
Apart from purely scientific uses, paper chromatogrphy is used for many applications. This is because it is cheap, relatively quick, and can be used to separate virtually any mixture. Often they are used to test for drugs in one's blood. Even CSI uses it to sequence DNA and RNA.
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